Britain’s Prince Harry and Meghan Markle to give up royal titles LATEST STORIES Takahashi gives PH its 2nd gold in judo, shocks Thai champ Trending Articles PLAY LIST 00:50Trending Articles02:11SEA GAMES 2019: PH’s Nesthy Petecio boxing featherweight final (HIGHLIGHTS)08:07Athletes treated to a spectacle as SEA Games 2019 officially ends06:27SEA Games 2019: No surprises as Gilas Pilipinas cruises to basketball gold05:02SEA Games 2019: Philippines clinches historic gold in women’s basketball05:21Drama in karate: Tsukii ‘very sad’ over coach’s bullying, cold shoulder03:24PH’s James Palicte boxing light welterweight final (HIGHLIGHTS) National Historical team rescues Amorsolos, artifacts from Taal Jake says relationship with Shaina ‘goes beyond physical attraction’ Hotdog’s Dennis Garcia dies She went on to become part of the national team, winning gold medal in the 2015 Shanghai Open.Her career reached another peak when she beat Amit for the gold in the Singapore SEA Games two years ago.Sports Related Videospowered by AdSparcRead Next Bishop Baylon encourages faithful in Albay to help Taal evacuees “I was rooting for McGregor, but he lost,” added the back-to-back SEA Games champion from Zamboanga City.On the pool table Sunday afternoon, she was the winner. But not before a big fright.FEATURED STORIESSPORTSEnd of his agony? SC rules in favor of Espinosa, orders promoter heirs to pay boxing legendSPORTSRedemption is sweet for Ginebra, Scottie ThompsonSPORTSMayweather beats Pacquiao, Canelo for ‘Fighter of the Decade’“I thought I lost the gold when she also went on a hill. I didn’t expect it to turn out that way,” said Centeno, who edged Amit, 7-6.READ: Centeno wins gold, Amit settles for silver anew in women’s 9-ball She said she has learned a lot from the two-time World 10-ball champion.“Champ (Amit) is a great player,” said said. “I learned a lot from her. Like her safety shots.”Fortunately for Centeno, Amit didn’t play safe in the 13th rack and gave away a clear table en route to the gold.READ: ‘Disappointed’ Amit says Centeno deserves the goldThe 18-year-old Centeno came in the spotlight when as an 11-year-old she competed in the Philippine National Games in Dumaguete in 2010.ADVERTISEMENT Police seize P68-M worth of ‘shabu’ in Pasay MOST READ Chezka Centeno and Rubilen Amit of the Philippines celebrate after winning gold and silver medals, respectively, in the women’s 9-ball singles event of the 29th Southeast Asian Games billiards competition Sunday at the Kuala Lumpur Convention Center. CONTRIBUTED PHOTO/SEA GAMES MEDIA POOLKUALA LUMPUR — Roommates here and close friends back home, Rubilen Amit and Chezka Centeno vowed that they never, ever, talked about their duel in the final.“We talked about many things, including the Mayweather-McGregor fight, but no, we didn’t talk about the final match,” said the soft-spoken Centeno in Filipino.ADVERTISEMENT Marcosian mode: Duterte threatens to arrest water execs ‘one night’ Britain’s Prince Harry and Meghan Markle to give up royal titles Search on for 5 Indonesians snatched anew in Lahad Datu Don’t miss out on the latest news and information. View comments
by Ross Anderson, Ph.D.I’m sure that most readers have heard of mitochondria, and how important they are to all eukaryotic cells. They are commonly referred to as the cell’s “powerhouse” as they are responsible for synthesizing the bulk of ATP required to power many reactions in a cell.A characteristic of all eukaryotic cells is that they have membrane-bound organelles within them. Some, like the nucleus and mitochondria, are bounded by two membranes, but most are bounded by a single membrane. Regardless of the number of membranes, all must have transport mechanism in place to get proteins into them in order to function properly. Each organelle utilizes different designs to transport proteins into them, or out of them.Two organelles, the endoplasmic reticulum (ER) and the mitochondria, have different complexes; however, there is one complex that is common to both. It is called Ubx2. A recent paper in Nature by Christoph U. Martensson et al., with a ho-hum title “Mitochondrial Protein Translocation-Associated Degradation,” is actually about quality control inside the cells of our bodies. The researchers found some exciting news about this protein complex Ubx2: it acts like a crossing guard, ferrying passengers safely through the membrane. But this is no ordinary crossing guard. It also acts as a bouncer or cop when bad proteins try to clog up the channel. And it has access to the whole search-and-rescue team. Without this entire system knowing what to do in advance of trouble, the whole cell could die.Making the IngredientsMaking ATP in the mitochondria requires the use of many different proteins that work as parts of large complexes. Some of these proteins are located in the matrix region of the mitochondria (the inner parts), and many are located between membranes, either the inner membrane or the outer membrane. A mitochondrion has a small genome of its own, but a large majority of its proteins are encoded by genes located in the nucleus—not in the mitochondrial DNA. Consequently, these genes must be transcribed in the nucleus, then translated into proteins in the cytosol of the cell (i.e., outside the mitochondria).Transporting the IngredientsAfter the precursor proteins have been transcribed in the cytosol, they must then be transported into the mitochondria and folded properly before they are able to function as proteins or enzymes. Because we live in a fallen world, not everything proceeds perfectly or is made perfectly; mutations occur. As such, sometimes proteins do not have the correct amino acid sequence, or even if they do, they simply don’t fold properly. Proteins destined for the mitochondria are maintained in an unfolded state until they are transported into the mitochondria where they are folded by special helper enzymes called chaperonins. This is accomplished by the binding of certain proteins in the cytosol known as heat-shock proteins (Hsp’s). Hsp’s are also called chaperones, because they bind to unfolded regions of proteins and prevent them from engaging in improper interactions that might lead to an improperly folded protein.Meet TOMIf mitochondrial protein precursors are not transported into the mitochondria properly, they will accumulate on the outer surface of the mitochondria. This leads to a mitochondrial-induced stress response by the cell whereby a number of cellular activities are affected. As a consequence of improper transport by the transport channel, TOM (Translocon Outer Membrane), becomes clogged and proteins can no longer be transported into the mitochondria. This can lead to down-regulation of mitochondrial proteins which will compromise the synthesis of ATP.The TOM itself is actually a complex of proteins. The various members, numbered like Tom20 and Tom70, work together for the efficient transport of mitochondrial proteins through the outer membrane. Each protein of the complex is encoded by its own gene, and the various genes are carefully regulated so that the correct number of components are ready for assembly of the TOM. As the name implies, TOM is located in the outer membrane and forms the channel through which proteins traverse the outer membrane. The TOM joins another protein complex, TIM (Translocon Inner Membrane), located in the inner membrane. TIM forms a channel through the inner membrane. Together these two complexes of proteins form a complete channel through both membranes. The TOM core complex is made up of Tom40, Tom22, and various small Tom proteins. This complex associates with Toms 20 and 70.The Quality Control TeamTogether, the proteins Ubx2 and Cdc48 promote clearance of stalled precursor proteins from the general entry gate of mitochondria, the TOM complex, for proteasomal degradation. Credit: Christoph Mårtensson, University of FreiburgThe authors found that Ubx2 associates with Tom40 and some of the other proteins of the complex, except for Tom70. Under conditions where a cell is stressed, another protein, Cis1 binds to Tom70 and recruits Msp1 which aids in the removal of non-imported precursor proteins. However, under ‘normal’ non-stressed conditions, Ubx2 binds. In the ER (endoplasmic reticulum), Ubx2 functions with two proteins, DOA10 and HRD1, which, together, recruit an ATPase (an ATP-powered enzyme), Cdc48, and function in the removal and degradation of proteins from channels in the ER membrane if they failed to make it through. So in the ER, Ubx2 associates with DOA10 and HRD1, but at the mitochondria, it has a different role. There, Ubx2 associates with Tom40, 22 and 20. This suggested to the authors that there must be two populations of Ubx2; one for the ER and one for mitochondria.Interestingly, Ubx2 itself, must be transported into the mitochondria in order to function. Once in the mitochondria, Ubx2 can associate with the mature Tom40 complex only in the presence of Tom22 and Tom70. Once associated with Tom40 the complex of Toms20, 22, 40 and 70 can recruit Cdc48 to the TOM complex. However, in order for Cdc48 to associate with the TOM there must be yet another two proteins, Ufd1 and Np14. If you can keep track of all this complexity, Cdc48 also has associated proteins, Vms1 and Msp1, required for mitochondrial-associated degradation of proteins!Putting it All TogetherA mitochondrion’s main function is to run its powerhouse, a sequence of molecular machines called the electron transport chain. This chain produces ATP from the food that we eat. The scientists in the Nature paper reported that, without Ubx2 and Vms1 or Msp1, the stability of the electron transport chain complexes (respiratory chain) in the inner membrane was compromised. As a result, proteins would get tagged with ubiquitin (the “kill-me” tag) for destruction and, as a result, would accumulate at the TOM. Thus, the TOM complex recruits Ubx2 which, in turn, recruits Cdc48 and its partner proteins, Udf1 and Np14. The Cdc48 complex then extracts arrested proteins from the TOM channel. The extracted proteins are then degraded by a proteasome (another complex of proteins that degrades faulty proteins and recycles the amino acids). The authors dubbed this quality control system ‘mitoTAD’ for mitochondrial translocation-associated degradation. Thus the “mitoTAD maintains the full functionality of the TOM complex for correct import of precursor proteins into the mitochondria.” Without it, aberrant precursor proteins would accumulate and clog the TOM. That, obviously, could lead to the detriment of the cell and the entire organism.Sticking to the ScienceMost likely, all the authors believe that evolution is the explanation for how this came to be. To their credit, though, they did not engage in evolutionary storytelling to try and prop up an evolutionary ideology. They clearly answered the three questions an objective scientist should ask:What is it?What does it do?How does it do it?It was refreshing, therefore, to see a paper stick to the science. That said, anyone reading this paper with an open mind would be amazed at the complex quality control involved in the seeming “simple” task of ushering proteins through a membrane. The transport systems usually work well, but it is evident that foresight was involved in knowing how to handle emergencies, like when protein precursors get stuck in the channel. Does this not all show clearly the hallmarks of intelligent design? Look how many proteins are required. Each of these must be able to bind to others, so that they function as a whole.Irreducible Complexity SquaredMutation studies by the authors showed that when one or more of the needed proteins are absent, the function of the whole is lost. Assembly of one complex of proteins is required before the proper assembling and functioning of another complex; e.g., assembly of the TOM complex sets the stage for recruitment of the Ubx2 complex which, in turn, sets the stage for recruitment of the Cdc48 complex. Thus, we see irreducible complexity at two points: an irreducibly complex collection of parts arranged in an irreducibly complex sequence over time.This is very much like a Rube Goldberg machine. (If you don’t know what that is, see this entertaining modern example on YouTube.) Clearly, one malfunction anywhere in the sequence stops everything downstream that depends on it. Nobody would think the device in the video would arise by chance. Let’s be consistent, therefore, and apply the same reasoning in this case. The inference to the best explanation therefore—arising from the scientific facts themselves—is that intelligence with foresight played a fundamental role in the creation of this system. Bible-believers like me joyfully attribute that mind to the wonder-working hand of the Creator God of Genesis. Recommended Resource: Foresight, a new book by Brazilian scientist Marcos Eberlin, gives numerous examples in nature of processes and systems that required planning and foresight to work. This pro-ID book has been endorsed by 3 Nobel laureates.(Visited 519 times, 1 visits today)FacebookTwitterPinterestSave分享0 Ross Anderson (PhD biochemistry) is professor of biochemistry at The Master’s University in southern California. Dr Anderson’s expertise is in the area of biochemistry and molecular biology. He has taught Biochemistry and helped to direct research projects of graduate and medical students at Baylor College of Medicine, Houston, TX. Dr. Anderson was a post-doctoral researcher in the Molecular Genetics Division of the Department of Ophthalmology at the Houston Neurosensory Center.Dr Anderson was a member of both the undergraduate and graduate faculty at Lamar University, Beaumont, TX. There he taught and directed the research activities of undergraduates and Masters of Science degree candidates in Biology. Currently he is professor of biochemistry at The Master’s University in southern California.Dr Anderson’s research interests include structure-function studies of DNA polymerizing enzymes and the synthesis and expression of synthetic human genes in bacterial hosts. He has authored or co-authored several publications in major, peer-reviewed journals. He is a member of the American Chemical Society and Sigma Xi Research Society.
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Growing Phone Scams: 5 Tips To Avoid Contracts for Java is a new open source tool from Google that makes it easier to implement contracts in Java. According to Google’s announcement, this new tool is based on Modern Jass by Johannes Rieken and inspired by the programming language Eiffel. It was created by two Google engineers – David Morgan, Andreas Leitner – using their 20% time, and was expanded as part of an internship by Nhat Minh Le.According to Wikipedia, Design by Contract is: An approach to designing computer software. It prescribes that software designers should define formal, precise and verifiable interface specifications for software components, which extend the ordinary definition of abstract data types with preconditions, postconditions and invariants. These specifications are referred to as “contracts”, in accordance with a conceptual metaphor with the conditions and obligations of business contracts.According to Google’s announcement:Traditionally, Java programmers enforced preconditions using explicit parameter validation code in public methods, and assertions in non-public methods. Likewise, they enforced invariants and postconditions using assertions. This approach is described in detail here. Since then, new features in Java 5 have enabled a more convenient and expressive implementation of contracts. klint finley How to Write a Welcome Email to New Employees? Related Posts Tags:#hack#Tools Why You Love Online Quizzes 7 Types of Video that will Make a Massive Impac…
marshall kirkpatrick The Dos and Don’ts of Brand Awareness Videos Facebook Deals Could Still Be HugeAll of the above might change in time but even at launch this is a very, very strong product. That vendors will recieve demographic and taste data about the people who redeem deals sounds incredibly valuable. That Deals will be social and will spread through the newsfeed does too.Some people have laughed that Facebook Deals is likely to “flop” like Facebook Places, Questions and Groups. Have those really flopped like a Google social software launch, though? Facebook says 50 million Groups have been created and that the feature is among the fastest-growing on the site. (Could that really be true, compared to Photos, Videos, Events, etc.? Maybe those are core features and don’t count.)Facebook Questions does seem to be less than thrilling in its traction. That’s a real shame – it had huge, huge potential but seemed poorly built.Places? Facebook says it’s already much, much bigger than competitors Foursquare and Gowalla say they are and at least one reputable survey of local businesses says that merchants are far more engaged with Facebook Places than other services. The Places feature is much simpler than it could be but perhaps (someday) integration with Deals will help with that.Unlike questions asked by random people, empty Places pages or strategically ambiguous Groups – Deals offers very clear value, created by professional marketers and vetted in part by your friends’ Likes and redemptions.Lots and lots of potential. A very interesting platform. Big caveats. Now it’s time to see how well the company can execute and how long it keeps itself slowed down by the above restrictions present at launch. Related Posts A Comprehensive Guide to a Content Audit Tags:#Analysis#Facebook#web Facebook is Becoming Less Personal and More Pro… It’s Not FreeLast night I reported that Facebook Deals could be free to merchants. It turns out that it’s only Facebook Check-in Deals that are free and those have been for a few months. Got that? Deals are not free, Check-in Deals are free. Deals other than Check-in Deals are not free. That would have been a very bold move on Facebook’s part and would have helped it undercut the whole market. The company could then have made initial profits from its cut of purchases made using Facebook Credits.But…Facebook Credits Will Not Be for Physical Goods at LaunchThe ability to make Deals purchases with the same Facebook Credits that users have paid to charge up to play games with seems like an incredible opportunity, though legally complicated. Perhaps that’s why Facebook PR wrote us back after the news of the feature launching leaked to clarify that Credits will not in fact be usable to purchase physical goods at launch. Rather, Credits can be used to purchase vouchers for things like events.It’s Not MobileThis part is a little amazing to me, but maybe the tangle of products and managers at Facebook warranted such a strategy: Facebook Deals will not be available through the mobile app at launch. That seems like a huge lost opportunity, but perhaps the Deals team didn’t want to step on the toes of the Check-in Deals team. Guide to Performing Bulk Email Verification News of a new coupon service from Facebook, called Facebook Deals, emerged last night and was unveiled early this morning. We looked at all that Facebook had to offer and said it could blow Groupon and Living Social out of the water.Now that the product has been unveiled and Facebook PR has sent us multiple emails letting us know that things I presumed would be features of the service will not in fact be present at launch – that changes things just a little bit. I still think that the fundamental value proposition of Facebook is clearly the strongest in the field: structured and verified user data, the friend network and the newsfeed. But there are a few details to clear up about the product and it looks like it’s going to be less ambitious at launch than I suspected last night. It still looks awesome, though.
Filmmakers and video pros need to know the difference between rolling shutter and global shutter. Here are the basics.An important part of the production process is understanding the difference between rolling shutter and global shutter. Both are distinct in terms of the final image result, especially when the camera is in motion. Let’s take a close look at the ins and outs of each and how they affect our final production.What Is Global Shutter?We’ll start with the cinematic standard of global shutter, which has more to do with a camera’s sensor than being a process or effect. A global shutter sensor would be a CCD sensor, however most of the digital cameras being used today — like the ARRI ALEXA — employ a CMOS sensor that utilizes global shutter to capture an entire frame all at once.Image via REDAs you see in the image above, the sensor is either on or it’s off. Digital cameras like the ARRI ALEXA utilize this feature when capturing imagery, which in turn allows the camera to capture all of the motion at once instead of progressively capturing motion like a rolling shutter. More on that shortly.Let’s take a quick look at the difference between a global shutter and a rolling shutter and the results yielded by having a global shutter capture all of the visual information at once.Video via SonyWhat Is Rolling Shutter?Image via WikipediaSo what is about rolling shutter that makes the blades of the helicopter in the video above bend and warp? Well, unlike global shutter where the sensor is exposed all at once, a rolling shutter is exposed in a progressive motion. You can see an example of this exposure in the image below and see how a rolling shutter is “always active and ‘rolling’ through pixels from top to bottom.” It is this progressive exposure that causes the warping. But as we’ve touched on before, there are ways to deal with rolling shutter in your footage.Image via SLRLoungeIt’s important to note that the rolling shutter effect often goes unnoticed, as long as the camera movement isn’t too chaotic. In fact, Blackmagic Design recently dropped global shutter in the URSA Mini 4.6K and decided to keep the rolling shutter instead. While this isn’t what Blackmagic ultimately wanted to do, it was an acceptable trade-off to ensure the 4.6K was able to reach up to fifteen stops of dynamic range. Also, keep in mind that you can utilize rolling shutter to your advantage in certain situations. For one such situation, check out the video (via Kyle Jones) below.A Quick OverviewAs you can see from the breakdowns above, there are stark differences between global and rolling shutter. However, if the information above isn’t enough (or a little difficult to fully grasp), then check out this clip from Videomaker. It offers simple examples of the differences between global shutter and rolling shutter.Was this breakdown helpful? Have any other cinematography techniques or terms you’d like to see broken down? Share your thoughts in the comments below.
While you’re here, we’d like you to consider subscribing to Pistols Firing and becoming a PFB+ member. It’s a big ask from us to you, but it also comes with a load of benefits like ad-free browsing (ads stink!), access to our premium room in The Chamber and monthly giveaways.The other thing it does is help stabilize our business into the future. As it turns out, sending folks on the road to cover games and provide 24/7 Pokes coverage like the excellent article you just read costs money. Because of our subscribers, we’ve been able to improve our work and provide the best OSU news and community anywhere online. Help us keep that up. AT&T Stadium has been selected as the site for the Big 12 Football Championship, the Conference announced Friday morning.AT&T Stadium to Host Big 12 Football Championship: https://t.co/3Zcu9Yj4aF— Darnell Mayberry (@DarnellMayberry) November 18, 2016The 100,000 seat stadium in Arlington, TX, will play host for the 2017-2021 seasons, where the top two teams in the conference will play each other (again) for the conference championship title. 2017 will feature the first Big 12 championship game since 2010.“There is great anticipation with the return of the Big 12 Football Championship and we look forward to hosting the event in AT&T Stadium for the next five years,” said Commissioner Bob Bowlsby in a release. “The building is a state of the art facility and offers many amenities that will be enjoyed by our institutions and fans.”FOX will cover the championship in 2017 and odd-numbered years through 2023, and ESPN will do the same in even-numbered years through 2024. Here are all the sites that have hosted the championship over the years.
Oklahoma State’s true freshman tailback Justice Hill’s emergence has catapulted him into an elite group. In Saturday’s game against TCU, he launched himself to No. 1 in rushing yards among OSU true freshmen, passing hall-of-famer Thurman Thomas in the process. He did so with this run.Here is the list of freshmen rushing leaders for OSU.Mike Hamilton (961 yards)Justice Hill (943 and counting)Jamaal Fobbs (928 yards)Thurman Thomas (843 yards)And here’s the list of true freshmen:This seems pretty good. pic.twitter.com/dSyAS8p3lf— Pistols Firing (@pistolsguys) November 19, 2016 While you’re here, we’d like you to consider subscribing to Pistols Firing and becoming a PFB+ member. It’s a big ask from us to you, but it also comes with a load of benefits like ad-free browsing (ads stink!), access to our premium room in The Chamber and monthly giveaways.The other thing it does is help stabilize our business into the future. As it turns out, sending folks on the road to cover games and provide 24/7 Pokes coverage like the excellent article you just read costs money. Because of our subscribers, we’ve been able to improve our work and provide the best OSU news and community anywhere online. Help us keep that up.